17 research outputs found
The mRNA-bound proteome of the early fly embryo
Early embryogenesis is characterized by the maternal to zygotic transition (MZT), in which maternally deposited messenger RNAs are degraded while zygotic transcription begins. Before the MZT, post-transcriptional gene regulation by RNA-binding proteins (RBPs) is the dominant force in embryo patterning. We used two mRNA interactome capture methods to identify RBPs bound to polyadenylated transcripts within the first two hours of D. melanogaster embryogenesis. We identified a high-confidence set of 476 putative RBPs and confirmed RNA-binding activities for most of 24 tested candidates. Most proteins in the interactome are known RBPs or harbor canonical RBP features, but 99 exhibited previously uncharacterized RNA-binding activity. mRNA-bound RBPs and TFs exhibit distinct expression dynamics, in which the newly identified RBPs dominate the first two hours of embryonic development. Integrating our resource with in situ hybridization data from existing databases showed that mRNAs encoding RBPs are enriched in posterior regions of the early embryo, suggesting their general importance in posterior patterning and germ cell maturation
Neutralino Dark Matter from MSSM Flat Directions in light of WMAP Result
The minimal supersymmetric standard model (MSSM) has a truly supersymmetric
way to explain both the baryon asymmetry and cold dark matter in the present
Universe, that is, ``Affleck-Dine baryo/DM-genesis.'' The associated late-time
decay of Q-balls directly connects the origins of the baryon asymmetry and dark
matter, and also predicts a specific nature of the LSP. In this paper, we
investigate the prospects for indirect detection of these dark matter
candidates observing high energy neutrino flux from the Sun, and hard positron
flux from the halo. We also update the previous analysis of the direct
detection in hep-ph/0205044 by implementing the recent result from WMAP
satellite.Comment: 32 pages, including 40 figure
Likelihood Functions for Supersymmetric Observables in Frequentist Analyses of the CMSSM and NUHM1
On the basis of frequentist analyses of experimental constraints from
electroweak precision data, g-2, B physics and cosmological data, we
investigate the parameters of the constrained MSSM (CMSSM) with universal soft
supersymmetry-breaking mass parameters, and a model with common non-universal
Higgs masses (NUHM1). We present chi^2 likelihood functions for the masses of
supersymmetric particles and Higgs bosons, as well as b to s gamma, b to mu mu
and the spin-independent dark matter scattering cross section. In the CMSSM we
find preferences for sparticle masses that are relatively light. In the NUHM1
the best-fit values for many sparticle masses are even slightly smaller, but
with greater uncertainties. The likelihood functions for most sparticle masses
are cut off sharply at small masses, in particular by the LEP Higgs mass
constraint. Both in the CMSSM and the NUHM1, the coannihilation region is
favoured over the focus-point region at about the 3-sigma level, largely but
not exclusively because of g-2. Many sparticle masses are highly correlated in
both the CMSSM and NUHM1, and most of the regions preferred at the 95% C.L. are
accessible to early LHC running. Some slepton and chargino/neutralino masses
should be in reach at the ILC. The masses of the heavier Higgs bosons should be
accessible at the LHC and the ILC in portions of the preferred regions in the
(M_A, tan beta) plane. In the CMSSM, the likelihood function for b to mu mu is
peaked close to the Standard Model value, but much larger values are possible
in the NUHM1. We find that values of the DM cross section > 10^{-10} pb are
preferred in both the CMSSM and the NUHM1. We study the effects of dropping the
g-2, b to s gamma, relic density and M_h constraints.Comment: 34 pages, 24 figure
Revisiting the Higgs Mass and Dark Matter in the CMSSM
Taking into account the available accelerator and astrophysical constraints,
the mass of the lightest neutral Higgs boson h in the minimal supersymmetric
extension of the Standard Model with universal soft supersymmetry-breaking
masses (CMSSM) has been estimated to lie between 114 and ~ 130 GeV. Recent data
from ATLAS and CMS hint that m_h ~ 125 GeV, though m_h ~ 119 GeV may still be a
possibility. Here we study the consequences for the parameters of the CMSSM and
direct dark matter detection if the Higgs hint is confirmed, focusing on the
strips in the (m_1/2, m_0) planes for different tan beta and A_0 where the
relic density of the lightest neutralino chi falls within the range of the
cosmological cold dark matter density allowed by WMAP and other experiments. We
find that if m_h ~ 125 GeV focus-point strips would be disfavoured, as would
the low-tan beta stau-chi and stop -chi coannihilation strips, whereas the
stau-chi coannihilation strip at large tan beta and A_0 > 0 would be favoured,
together with its extension to a funnel where rapid annihilation via
direct-channel H/A poles dominates. On the other hand, if m_h ~ 119 GeV more
options would be open. We give parametrizations of WMAP strips with large tan
beta and fixed A_0/m_0 > 0 that include portions compatible with m_h = 125 GeV,
and present predictions for spin-independent elastic dark matter scattering
along these strips. These are generally low for models compatible with m_h =
125 GeV, whereas the XENON100 experiment already excludes some portions of
strips where m_h is smaller.Comment: 24 pages, 9 figure
Energy and system size dependence of \phi meson production in Cu+Cu and Au+Au collisions
We study the beam-energy and system-size dependence of \phi meson production
(using the hadronic decay mode \phi -- K+K-) by comparing the new results from
Cu+Cu collisions and previously reported Au+Au collisions at \sqrt{s_NN} = 62.4
and 200 GeV measured in the STAR experiment at RHIC. Data presented are from
mid-rapidity (|y|<0.5) for 0.4 < pT < 5 GeV/c. At a given beam energy, the
transverse momentum distributions for \phi mesons are observed to be similar in
yield and shape for Cu+Cu and Au+Au colliding systems with similar average
numbers of participating nucleons. The \phi meson yields in nucleus-nucleus
collisions, normalised by the average number of participating nucleons, are
found to be enhanced relative to those from p+p collisions with a different
trend compared to strange baryons. The enhancement for \phi mesons is observed
to be higher at \sqrt{s_NN} = 200 GeV compared to 62.4 GeV. These observations
for the produced \phi(s\bar{s}) mesons clearly suggest that, at these collision
energies, the source of enhancement of strange hadrons is related to the
formation of a dense partonic medium in high energy nucleus-nucleus collisions
and cannot be alone due to canonical suppression of their production in smaller
systems.Comment: 20 pages and 5 figure
Collider aspects of flavour physics at high Q
This review presents flavour related issues in the production and decays of
heavy states at LHC, both from the experimental side and from the theoretical
side. We review top quark physics and discuss flavour aspects of several
extensions of the Standard Model, such as supersymmetry, little Higgs model or
models with extra dimensions. This includes discovery aspects as well as
measurement of several properties of these heavy states. We also present public
available computational tools related to this topic.Comment: Report of Working Group 1 of the CERN Workshop ``Flavour in the era
of the LHC'', Geneva, Switzerland, November 2005 -- March 200
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts
Protein-RNA interactions are fundamental to core biological processes, such as mRNA splicing, localization, degradation, and translation. We developed a photoreactive nucleotide-enhanced UV crosslinking and oligo(dT) purification approach to identify the mRNA-bound proteome using quantitative proteomics and to display the protein occupancy on mRNA transcripts by next-generation sequencing. Application to a human embryonic kidney cell line identified close to 800 proteins. To our knowledge, nearly one-third were not previously annotated as RNA binding, and about 15% were not predictable by computational methods to interact with RNA. Protein occupancy profiling provides a transcriptome-wide catalog of potential cis-regulatory regions on mammalian mRNAs and showed that large stretches in 3' UTRs can be contacted by the mRNA-bound proteome, with numerous putative binding sites in regions harboring disease-associated nucleotide polymorphisms. Our observations indicate the presence of a large number of mRNA binders with diverse molecular functions participating in combinatorial posttranscriptional gene-expression networks